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Synthesis and high-performance liquid chromatographic purification of tritiated thyrotrophin-releasing hormone-like peptides

✍ Scribed by R. Bílek; A.F. Bradbury; D.G. Smyth


Book ID
103804977
Publisher
Elsevier Science
Year
1994
Weight
352 KB
Volume
656
Category
Article
ISSN
0378-4347

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Amunine, or corticotropin releasing factor (CRF), a 41-peptide amide, has been isolated from ovine hypothalami. Tissues were extracted, defatted, and partitioned prior to being sized by gel permeation. The active zone followed by a sensitive in vitro assay (CRF-mediated corticotropin release from pi