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Synthesis and expression of the gene for human epidermal growth factor in transgenic potato plants

✍ Scribed by A-H. Salmanian; A. Gushchin; T. Medvedeva; M. R. Noori-Daloii; N. Domansky

Publisher
Springer Netherlands
Year
1996
Tongue
English
Weight
238 KB
Volume
18
Category
Article
ISSN
0141-5492

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✦ Synopsis

The gene for human epidermal growth factor (hEGF) was chemically synthesized and used for expression in transgenic potato. The hEGF coding sequence was modified by PCR to introduce ATG start codon and fused either to the 35S cauliflower mosaic virus promoter or to the patatin class I promoter. The highest hEGF peptide content, 120 pg/mg soluble protein, was found in potato tubers when the chimeric gene was expressed under the control of patatin promoter.

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