Synthesis and export of tem1 β-lactamase in escherichia coli
✍ Scribed by Daniel Baty; Alain Bernadac; Yolande Berthois; Claude Lazdunski
- Book ID
- 115912126
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 717 KB
- Volume
- 127
- Category
- Article
- ISSN
- 0014-5793
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The lipase (lip) gene of Staphylococcus hyicus was used to study the expression of the Escherichia coli beta-lactamase (bla) gene in S. carnosus. The bla gene, devoid of its promotor and most of the signal sequence, was fused to the lip structural gene at various positions. A set of 11 secretion vec
A mutant of Escherichia coli, in which signal peptidase I synthesis can be regulated, was constructed. The mutant was used to study the effects of signal peptidase I limitation on the synthesis and efficiency of processing of two proteins: the periplasmic E. coli TEM-beta-lactamase and Bacillus lich