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Synthesis and enzymatic incorporation of α-l-threofuranosyl adenine triphosphate (tATP)

✍ Scribed by Zhang, Su; Chaput, John C.


Book ID
122906833
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
248 KB
Volume
23
Category
Article
ISSN
0960-894X

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4-(carboxymethyl)-2-(thymidin-9-yl)-6-(hydro~methyl)morpholine~-triphosphate (morpholino thymidine-5'-triphosphate) was synthesised from 1 -(PD-erythro-pentofuranosyl) thymine. It was fully characterised by NMR, UV and mass spectrometry. Taq polymerase enzymatic incorporation of this nucleotide anal