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Synthesis and Deprotection of Oligonucleotides under Aprotic Conditions

โœ Scribed by Chen, Tongqian; Fu, Jiasheng; Greenberg, Marc M.


Book ID
127305586
Publisher
American Chemical Society
Year
2000
Tongue
English
Weight
57 KB
Volume
2
Category
Article
ISSN
1523-7060

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We have developed methylaminelammonia as a fast cleavage and deprotection reagent which effects complete cleavage of oligonucleotides from the solid support in 5 min at room temperature and complete deprotection in 5 min at 65ยฐC. The problem of transamination side product formation, faced with the c