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Fast cleavage and deprotection of oligonucleotides

✍ Scribed by M.P. Reddy; N.B. Hanna; Firdous Farooqui

Book ID
104214559
Publisher
Elsevier Science
Year
1994
Tongue
French
Weight
256 KB
Volume
35
Category
Article
ISSN
0040-4039

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✦ Synopsis

We have developed methylaminelammonia as a fast cleavage and deprotection reagent which effects complete cleavage of oligonucleotides from the solid support in 5 min at room temperature and complete deprotection in 5 min at 65Β°C. The problem of transamination side product formation, faced with the commonly used dC" (10.0% side product) upon treatment with methylamine/ammonia, has been successfully solved by the use of dCr (0.0% side product). DMT dC" phosphoramidiie -methylaminelammonia system furnished oligonucleotides in equal or superior quality as compared to the other systems.

πŸ“œ SIMILAR VOLUMES

ChemInform Abstract: Cleavage and Deprot
ChemInform Abstract: Cleavage and Deprotection
✍ Henning Lund πŸ“‚ Article πŸ“… 2010 πŸ› John Wiley and Sons βš– 22 KB

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