Synthesis and degradation of adenosine triphosphate in cod (Gadus morhua) at subzero temperatures

✍ Scribed by Cappeln, Gertrud; Nielsen, Jette; Jessen, Flemming

• Book ID: 101225499
• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 111 KB
• Volume: 79
• Category: Article
• ISSN: 0022-5142
• DOI: 10.1002/(sici)1097-0010(199906)79:8<1099::aid-jsfa332>3.0.co;2-#

✦ Synopsis

This study has demonstrated that the extraction step is very important when analysing ATP and its degradation products. An important factor is whether the sample is fresh, frozen or thawed when homogenised since thawing of the sample will lead to rapid loss of ATP. During frozen storage it was found that ATP in cod (Gadus morhua) was stable at À40 °C in small samples for at least 12 weeks. At À20 °C it was found that ATP content increases initially and thereafter falls. It was demonstrated that degradation of ATP in small samples occurs faster at 0 °C than at À2 and À5 °C. Furthermore, it was found that in whole cod ATP could be synthesised at a signi®cant rate at À7 °C.