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Synthesis and Characterization of Silica-zirconia Composite for the Enrichment of Phosphopeptides

✍ Scribed by Jian-Hong WU; Kuang XIAO; Yong ZHAO; Wei-Ping ZHANG; Lin GUO; Yu-Qi FENG

Publisher
Elsevier Science
Year
2010
Tongue
Chinese
Weight
577 KB
Volume
38
Category
Article
ISSN
1872-2040

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✦ Synopsis

Silica-zirconia composite (SiO 2 -ZrO 2 ) was synthesized by sol-gel method, and its physical-chemical properties were investigated using several techniques. The enrichment capacity of silica-zirconia composite and zirconia was compared using tryptic digest of α-casein as probe. The results showed that the selectivity of SiO 2 -ZrO 2 was better than that of ZrO 2 when the pH of the loading solution was below 2. The enrichment capacity of SiO 2 -ZrO 2 for multiple phosphorylated peptides was better than that of ZrO 2 . In combination with matrix-assisted laser desorption ionization-time of flight (MALDI-TOF)-based peptide mass finger printing technique, SiO 2 -ZrO 2 was used to enrich the phosphopeptides from nonfat milk successfully.

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