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Preparation and characterization of ceria-zirconia composite for enrichment and identification of phosphopeptides

✍ Scribed by Jian-Hong Wu; Kuang Xiao; Yong Zhao; Wei-Ping Zhang; Lin Guo; Yu-Qi Feng

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
339 KB
Volume
33
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

Ceria‐zirconia composites at different molar ratios were synthesized. Several methods were used to characterize these composites, including X‐ray photoelectron spectroscopy, surface area and surface acid‐base property detection. A one‐step method for isolation and identification of phosphopeptides from peptide mixture was created using these ceria‐zirconia composites. Using tryptic digest of standard phosphorylated protein, we have shown that these enrichment and dephosphorylation activities are effective. The adsorption capacity and catalytic property of ceria‐zirconia composites at different molar ratios and calcinated temperatures were studied. In combination with MALDI‐TOF‐based peptide mass finger printing technique, we have established a method to utilize the enrichment/dephosphorylation dual properties of these ceria‐zirconia composites for the analysis of phosphoprotein in nonfat milk successfully.

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