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Synthesis and Characterization of an Immobilized Phenylethanolamine N-Methyltransferase Liquid Chromatographic Stationary Phase

✍ Scribed by Nektaria Markoglou; Irving W. Wainer

Publisher: Elsevier Science
Year: 2001
Tongue: English
Weight: 82 KB
Volume: 288
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.2000.4884

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✦ Synopsis

Norepinephrine is N-methylated to epinephrine by the catalytic effect of the terminal enzyme in catecholamine biosynthesis, phenylethanolamine N-methyltransferase (PNMT). PNMT has been covalently immobilized onto a silica-based liquid chromatographic support, glutaraldehyde-P (Glut-P). The resulting PNMT-Glut-P stationary phase (PNMT-SP) was enzymatically active, stable, and reusable. Standard Michaelis-Menten kinetic studies were performed with both free and immobilized PNMT and known substrates and inhibitors were examined. The results demonstrate that the PNMT-SP can be utilized for the rapid screening of potential PNMT substrates as well as the screening of compounds for PNMT inhibitory activity.

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