Application of an enzyme-based stationary phase to the determination of enzyme kinetic constants and types of inhibition: New high-performance liquid chromatographic approach utilizing an immobilized artificial membrane chromatographic support

Enzyme-based high-performance liquid chr

Enzyme-based high-performance liquid chromatography supports as probes of enzyme activity and inhibition: The immobilization of trypsin and α-chymotrypsin on an immobilized artificial membrane high-performance liquid chromatography support

✍ Wai-Keung Chui; Irving W. Wainer 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 939 KB

Immobilized artificial membrane (IAM) HPLC supports have been used to immobilize the enzymes alpha-chymotrypsin and trypsin. The enzymes were trapped in hydrophobic cavities on the support and were not covalently attached to the IAM surface. The resulting IAM-enzyme supports retained the hydrolytic