N,Nะ-Dicyanomethyl-o-phenylenediamine was synthesized with a 90% yield by a reaction of o-phenylenediamine with chloroacetonitrile in triethylamine. Our experimental results showed that it was the effective fluorogenic substrate for horseradish peroxidase (HRP) and hemin. The K m for the HRP system
Synthesis and characterization of a new fluorogenic substrate for alpha-galactosidase
โ Scribed by Zhen-Dan Shi; Omid Motabar; Ehud Goldin; Ke Liu; Noel Southall; Ellen Sidransky; Christopher P. Austin; Gary L. Griffiths; Wei Zheng
- Book ID
- 105892449
- Publisher
- Springer
- Year
- 2009
- Tongue
- English
- Weight
- 410 KB
- Volume
- 394
- Category
- Article
- ISSN
- 1618-2650
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The synthesis of a fluorescein-derived fluorogenic substrate, D,L-myo-inositol 1- ([6'-hexyloxy-3-oxo]spiro[isobenzofuran-l(3H),9'-[9H]xanthen]-3'-yl hydrogen phosphate) sodium salt 9, for assay of activity of phosphatidylinositide-specific phospholipase C (PI-PLC), is described. This substrate is s