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Synthesis of a new fluorogenic substrate for the assay of phosphoinositide-specific phospholipase C

✍ Scribed by Aleksey V. rukavishnikov; Miles P. Smith; G. Bruce Birrell; John F.W. Keana; O. Hayes Griffith

Publisher
Elsevier Science
Year
1998
Tongue
French
Weight
213 KB
Volume
39
Category
Article
ISSN
0040-4039

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✦ Synopsis

The synthesis of a fluorescein-derived fluorogenic substrate, D,L-myo-inositol 1- ([6'-hexyloxy-3-oxo]spiro[isobenzofuran-l(3H),9'-[9H]xanthen]-3'-yl hydrogen phosphate) sodium salt 9, for assay of activity of phosphatidylinositide-specific phospholipase C (PI-PLC), is described. This substrate is shown to be a sensitive substrate for bacterial PI-PLC in a continuous, non-radioactive assay.

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