𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Synthesis and characterization of a chimeric peptide derived from fasciculin that inhibits acetylcholinesterase

✍ Scribed by Roberto J. Falkenstein; Germán G. Gornalusse; Dr Clara Peña

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
180 KB
Volume
10
Category
Article
ISSN
1075-2617

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Fasciculins are peptides isolated from mamba (Dendroaspis) venoms which exert their toxic action by inhibiting acetylcholinesterase (AChE). They contain a characteristic triple stranded antiparallel β‐sheet formed by residues 22–27, 34–39 and 48–53. A chimeric peptide named Fas‐C, encompassing most of these sequences was synthesized using SPPS/Boc‐chemistry and characterized chemically, structurally and functionally. Fas‐C has two disulfide bridges, formed sequentially using dual cysteine protection.

SDS‐PAGE patterns, HPLC profiles and MS proved the peptide identity. Circular dichroism indicated the presence of 13.6% and 41.6% of β‐sheet and β‐turn, respectively, comparable to values observed in the native toxin. An inhibitory effect on eel AChE was displayed by the peptide (K~i~71.6 ± 18.3 µ M), although not reaching the affinity level of the parent native toxin (K~i~ 0.3 nM). It is confirmed that the principal binding region of fasciculin to AChE resides within loop II. Copyright © 2004 European Peptide Society and John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

Synthesis and characterization of amino
Synthesis and characterization of amino protected peptides derived from amino-α- hydroxyiminophosphonates
✍ Eli Breuer; Muhammad Safadi; Michael Chorev; Adam Vineze; Peter Bel 📂 Article 📅 1991 🏛 Elsevier Science 🌐 French ⚖ 408 KB

Diisopropyl 2-amino-l-hydroxyiminoethylphosphonate (2\_a), derived from glycine, and diisopropyl 4-amino-l-hydroxyiminobutylphosphonate (2\_\_~), derived from GABA, were reacted with Boc-L-Phe-OH by the mixed anhydride method to yield novel peptide analogs diisopropyl 2-(t-Boc-L-Phe)amino-l-hydroxyi

Synthesis, Characterization and Conforma
Synthesis, Characterization and Conformational Analysis of gp120-derived Synthetic Peptides That Specifically Enhance HIV-1 Infectivity
✍ Monica Dettin; Rossella Roncon; Mario Simonetti; Silvia Tormene; Lucia Falcigno; 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 162 KB 👁 2 views

A series of peptides patterned on the principal neutralizing domain of the HIV-1 envelope glycoprotein gp 120 have been synthesized by solid-phase techniques. Interestingly, in vitro experiments have shown that some of these peptides specifically interact with CD4 and, in particular, that the peptid