✦ LIBER ✦

Synthesis and characterization by fast atom bombardment mass spectrometry of peptides related to the B-domain of staphylococcal protein A

✍ Scribed by R. Wait; B. James; M. R. Calder

Publisher: John Wiley and Sons
Year: 1991
Tongue: English
Weight: 500 KB
Volume: 26
Category: Article
ISSN: 1076-5174
DOI: 10.1002/oms.1210260516

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✦ Synopsis

Solid-phase synthesis was used to prepare several peptides related to staphylococcal protein A, including a 58residue sequence corresponding to the entire &domain of the protein. These materials were characterized by a combination of fast atom bombardment mass spectrometry and microchemical methods, including Edman degradation and tryptic digestion. The errors identified included incomplete removal of blocking groups, premature chain termination and the dehydration of an aspartic acid residue in the sequence. All of these problems would have been extremely dilfcult to elucidate by conventional techniques of protein chemistry, and demonstrate that mass spectrometry is the method of choice for verifying the structural integrity of the products of solid-phase synthesis.

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