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Syntheses and testing of substrates and mechanism-based inactivators for xylanases

✍ Scribed by Lothar Ziser; Ika Setyawati; Stephen G. Withers

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
876 KB
Volume
274
Category
Article
ISSN
0008-6215

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✦ Synopsis

The syntheses of the 2,5-and 3,4-dinitrophenyl /3-xylobiosides by two separate routes are described, as well as the syntheses of the 2,4-dinitrophenyl fl-glycosides of 2-chloro-2-deoxyxylobiose and 2-deoxy-2-fluoro-xylobiose. Both the 3,4-and 2,5-dinitrophenyl fl-xylobiosides proved to be good substrates for the Bacillus subtilis xylanase, with keat/K m values of 1.0 and 34.4 mM-I s-l, respectively. Excellent time-dependent inactivation of the exoxylanase/glucanase from Cellulomonas fimi was provided by 2,4-dinitrophenyl 2-deoxy-2-fluoro-3-xylobioside, according to inactivation parameters of k i = 0.057 min-1 and K i = 0.0035 mM.

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