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Synchronous cell divisions in Anacystis nidulans Richter

โœ Scribed by Lorenzen, Harald ;Venkataraman, G. S.


Publisher
Springer-Verlag
Year
1969
Weight
231 KB
Volume
67
Category
Article
ISSN
0003-9276

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โœฆ Synopsis


The blue-green alga, Anacystis nidulans was successfully synchronized in continuous light (15,000 lux) by a 14 hr cycle, consisting of 8 hrs at 26 ~ C, and 6 hrs at 32 ~ C, coupled with periodic dilution of the algal suspension to a constant cell number at the end of each cycle. The alga continues to grow at the lower temperature, and a "division burst" begins 2 hrs after transfer to the higher temperature.

Zusammen

/assung. Die Cyanophyeee Anacystis nidulans wurde in Dauerlicht yon 15000Lux vollsti~ndig synchronisiert. Die Cyclen bestehen aus 8 Std bei 26 ~ C und 6 Std bei 32 ~ C, an ihrem Ende wurde jeweils mit frischer ~hrlSsung auf die Ansgangszellzahl yon 3-107 Zellen/ml verdiinnt. Bei beiden Temperaturen kann sich die Alge vermehren, unter den synehronisierenden Bedingungen des Temperaturwechsels beginnt der Teilungsschub (Verdopplung der Zellzahl) bei 32 ~ C nach 2 Std.


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