Following the induction of synchronous growth of Anacystis nidulans by light and C02 deprivation, cell mass and I~NA and DNA content during two cell cycles were measured. Both RNA and DNA synthesis were discontinuous and marked variation in survival to ultraviolet light was related to the state of r
Synchronous cell divisions in Anacystis nidulans Richter
โ Scribed by Lorenzen, Harald ;Venkataraman, G. S.
- Publisher
- Springer-Verlag
- Year
- 1969
- Weight
- 231 KB
- Volume
- 67
- Category
- Article
- ISSN
- 0003-9276
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โฆ Synopsis
The blue-green alga, Anacystis nidulans was successfully synchronized in continuous light (15,000 lux) by a 14 hr cycle, consisting of 8 hrs at 26 ~ C, and 6 hrs at 32 ~ C, coupled with periodic dilution of the algal suspension to a constant cell number at the end of each cycle. The alga continues to grow at the lower temperature, and a "division burst" begins 2 hrs after transfer to the higher temperature.
Zusammen
/assung. Die Cyanophyeee Anacystis nidulans wurde in Dauerlicht yon 15000Lux vollsti~ndig synchronisiert. Die Cyclen bestehen aus 8 Std bei 26 ~ C und 6 Std bei 32 ~ C, an ihrem Ende wurde jeweils mit frischer ~hrlSsung auf die Ansgangszellzahl yon 3-107 Zellen/ml verdiinnt. Bei beiden Temperaturen kann sich die Alge vermehren, unter den synehronisierenden Bedingungen des Temperaturwechsels beginnt der Teilungsschub (Verdopplung der Zellzahl) bei 32 ~ C nach 2 Std.
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