A lipopolysaccharide was isolated from cell walls of Anacystis nidulans by extraction with 450/0 aqueous phenol at 65 ~ and further purified by repeated high speed eentrifugation. It contains 30--400/0 of lipid and about 60o/0 of carbohydrate components. The carbohydrate moiety contains predominantl
Synchronous growth and genome replication in the blue-green alga Anacystis nidulans
โ Scribed by Herdman, M. ;Faulkner, B. M. ;Carr, N. G.
- Publisher
- Springer-Verlag
- Year
- 1970
- Weight
- 689 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0003-9276
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โฆ Synopsis
Following the induction of synchronous growth of Anacystis nidulans by light and C02 deprivation, cell mass and I~NA and DNA content during two cell cycles were measured. Both RNA and DNA synthesis were discontinuous and marked variation in survival to ultraviolet light was related to the state of replication. A model is presented which accounts for the proportion of cells (66~ induced into synchronous genome replication which is also related to the state of replication at the onset of pre-synckrony treatment.
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## Abstract The glutamate and glutamine pool contains about 75% of the free amino acids of __Anacystis nidulans__ harvested from a growing culture. Light pulses induced oscillations of the rate of oxygen uptake and of the size of the glutamate pool under certain physiological conditions. The freque