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Synchronization in the cell cycle by inhibitors of DNA replication induces histone H2AX phosphorylation: an indication of DNA damage

✍ Scribed by A. Kurose; T. Tanaka; X. Huang; F. Traganos; Z. Darzynkiewicz


Book ID
108677707
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
231 KB
Volume
39
Category
Article
ISSN
0960-7722

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## Abstract ## Background The induction of DNA double‐strand breaks (DSBs) in chromatin triggers histone H2AX phosphorylation (on __Ser__‐139) by ATM‐, ATR‐, or DNA‐dependent protein kinases (DNA‐PK). Phosphorylated H2AX, denoted as Ξ³H2AX, can be detected immunocytochemically using an antibody tha