Symmetric Behavior of Hemoglobin α- and β- Subunits during Acid-Induced Denaturation Observed by Electrospray Mass Spectrometry †

Observation of symmetric denaturation of

Observation of symmetric denaturation of hemoglobin subunits by electrospray ionization mass spectrometry

✍ Xian Wang; Wenjie Zhao; Xin Lin; Bo Su; Jianfeng Liu 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 276 KB

## Abstract Electrospray ionization mass spectrometry (ESI‐MS) has been used to characterize the denaturation of porcine hemoglobin (Hb) induced by solvent changes. This work provides evidence for the symmetric nature of Hb denaturation and demonstrates that heme losses from α‐ and β‐monomers occur