SV40-Specific RNA in the Nucleus and Polyribosomes of Transformed Cells
โ Scribed by Uno Lindberg and J. E. Darnell
- Book ID
- 123649227
- Publisher
- National Academy of Sciences
- Year
- 1970
- Tongue
- English
- Weight
- 410 KB
- Volume
- 65
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/59564
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๐ SIMILAR VOLUMES
Rapidly labelled nuclear RNA from an SV40 transformed mouse 3T3 line was isolated, and different molecular size classes pooled. The fractions were treated with Ehrlich ascites exonuclease III for various lengths of time, and the digestion of the 3' ends of the RNA measured by the appearance of TCA-s
The question about the nature of promoters in the transcriptional units containing SV-40 sequences in transformed cells was analyzed. It was found that the pulse-labeled RNA hybridizing to SV-40 DNA contains small but significant amounts of triphosphorylated 5 '-ends detected as pppGp in alkaline hy