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SV40-Mediated Immortalization

✍ Scribed by Krishna K. Jha; Satnam Banga; Vaseem Palejwala; Harvey L. Ozer

Book ID
115603096
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
93 KB
Volume
245
Category
Article
ISSN
0014-4827

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Normal human diploid fibroblasts (HF) have a limited life span, undergo senescence, and rarely, if ever, spontaneously immortalize in culture. Introduction of the gene for T antigen encoded by the DNA virus SV40 extends the life span of HF and increases the frequency of immortalization; however, imm