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Surface Structure and Coverage of an Oligonucleotide Probe Tethered onto a Gold Substrate and Its Hybridization Efficiency for a Polynucleotide Target

โœ Scribed by Huang, Eva; Satjapipat, Munlika; Han, Shubo; Zhou, Feimeng


Book ID
126436189
Publisher
American Chemical Society
Year
2001
Tongue
English
Weight
511 KB
Volume
17
Category
Article
ISSN
0743-7463

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Using a fluorescence-based method, we have determined the number of thiol-derivatized single-stranded oligonucleotides bound to gold nanoparticles and their extent of hybridization with complementary oligonucleotides in solution. Oligonucleotide surface coverages of hexanethiol 12-mer oligonucleotid