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A Fluorescence-Based Method for Determining the Surface Coverage and Hybridization Efficiency of Thiol-Capped Oligonucleotides Bound to Gold Thin Films and Nanoparticles

โœ Scribed by Demers, Linette M.; Mirkin, Chad A.; Mucic, Robert C.; Reynolds, Robert A.; Letsinger, Robert L.; Elghanian, Robert; Viswanadham, Garimella


Book ID
118737748
Publisher
American Chemical Society
Year
2000
Tongue
English
Weight
87 KB
Volume
72
Category
Article
ISSN
0003-2700

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A Fluorescence-Based Method for Determin
โœ Demers, Linette M.; Mirkin, Chad A.; Mucic, Robert C.; Reynolds, Robert A.; Lets ๐Ÿ“‚ Article ๐Ÿ“… 2000 ๐Ÿ› American Chemical Society ๐ŸŒ English โš– 87 KB

Using a fluorescence-based method, we have determined the number of thiol-derivatized single-stranded oligonucleotides bound to gold nanoparticles and their extent of hybridization with complementary oligonucleotides in solution. Oligonucleotide surface coverages of hexanethiol 12-mer oligonucleotid