Surface plasmon resonance and impedance spectroscopy on gold electrode for biosensor application

✍ Scribed by S. Helali; H. Ben Fredj; K. Cherif; A. Abdelghani; C. Martelet; N. Jaffrezic-Renault

• Book ID: 103847325
• Publisher: Elsevier Science
• Year: 2008
• Tongue: English
• Weight: 427 KB
• Volume: 28
• Category: Article
• ISSN: 0928-4931
• DOI: 10.1016/j.msec.2007.10.065

✦ Synopsis

This paper describes new-label free immunoassays for the detection of atrazine using optical Surface Plasmon Resonance (SPR) and Electrochemical Impedance Spectroscopy (EIS) by employing gold functionalised with self-assembled mixed monolayer. The mixed monolayer, formed by 16-mercaptohexadecanoic acid (MHDA) and biotinyl-PE, is used as the basic layer in the fabrication of the immunoprobe. The interfacial interaction of a biotinyl-Fab fragment K47 antibody with the mixed monolayer is based on a biotin-avidin system using the neutravidin molecule. Thus, in this study the multilayer engineering is mentored by SPR and EIS methods. In addition, the specificity and sensitivity of this self-assembled multilayer system to the presence of atrazine are investigated. The limit detection of Atrazine obtained with EIS technique is equal to 20 ng/ml and with SPR technique is equal to 50 ng/ml.