Development and Application of Surface Plasmon Resonance-Based Biosensors for the Detection of Cell–Ligand Interactions

✍ Scribed by John G. Quinn; Shane O'Neill; Aidan Doyle; Colm McAtamney; Dermot Diamond; Brian D. MacCraith; Richard O'Kennedy

• Publisher: Elsevier Science
• Year: 2000
• Tongue: English
• Weight: 141 KB
• Volume: 281
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.2000.4564

✦ Synopsis

Surface plasmon resonance (SPR)-based biosensors

were investigated with a view to providing a portable, inexpensive alternative to existing technologies for "real-time" biomolecular interaction analysis of whole cell-ligand interactions. A fiber optic SPR-based (FOSPR) biosensor, employing wavelength-dependent SPR, was constructed to enable continuous real-time data acquisition. In addition, a commercially available integrated angle-dependent SPR-based refractometer (ISPR) was modified to facilitate biosensing applications. Solid-phase detection of whole red blood cells (RBCs) using affinity-captured blood group specific antibodies was demonstrated using the BIACORE 1000, BIACORE Probe, FOSPR, and ISPR sensors. Nonspecific binding of RBCs to the hydrogel-based biointerface was negligible. However, the background noise level of the FOSPR-based biosensor was ϳ25-fold higher than that of the widely used BIACORE 1000 system while that of the ISPR-based biosensor was over 100-fold higher. Nevertheless, the FOSPR biosensor was suitable for the analysis of macromolecular analytes contained in crude matrices.