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Surface membrane differentiation of hemopoietic cells as observed by radioactive labeling

✍ Scribed by M. Fehlmann; L. Lafleur; N. Marceau

Publisher
John Wiley and Sons
Year
1977
Tongue
English
Weight
686 KB
Volume
90
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

Bone marrow cells from normal rats were separated by velocity sedimentation into three distinct populations corresponding to granulocytes, lymphocytes and reticulocytes. Cells from each population were surface labelled via the lactoperoxidase radioiodination or the tritiated borohydride reduction. Distinct labeling patterns were observed on SDS‐PAGE for each cell population. Separation of bone marrow cells from anemic rats injected with TAB vaccine led to four populations corresponding to successive stages of erythroid cell maturation. Labelled protein patterns were not in this case as different from one population to the other, except for one species which increased in intensity with the degree of maturation. The tritiated glycoprotein profiles show a shift from high to low molecular weight species during the process of maturation.

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