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Surface markers on human B- and T-lymphocytes. IX. Two-color immunofluorescence studies on the association between EBV receptors and complement receptors on the surface of lymphoid cell lines

✍ Scribed by Eitan Yefenof; George Klein; Mikael Jondal; Michael B. A. Oldstone

Publisher
John Wiley and Sons
Year
1976
Tongue
French
Weight
788 KB
Volume
17
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

Receptors for the third component of complement (C3) were demonstrated on the surface of established human lymphoid cell lines by a membrane fluorescence test with FITC‐ or TRITC‐conjugated antibodies against human C3. Two‐color fluorescence staining of EBV receptors and C3 receptors showed complete overlapping of green and red fluorescence. Capping of the EBV receptor induced co‐capping of the C3 receptor and vice versa. There was neither overlapping nor co‐capping when EBV or C3 receptors were examined in relation to Fc receptors, surface IgM or β~2~ microglobulin. The kinetic pattern of EBV receptor capping was identical with the pattern of C3 receptor capping but differed from the pattern of IgM capping. These results suggest a close association between EBV and C3 receptors on the human B‐lymphocyte.

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Membrane receptor stripping confirms the
Membrane receptor stripping confirms the association between ebv receptors and complement receptors on the surface of human b lymphoma lines
✍ Eitan Yefenof; George Klein 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 French ⚖ 434 KB

## Abstract Complement (C3) receptors, EBV receptors, Fc receptors, membrane IgM and beta~2~microglobulin (β~2~m) were individually stripped from the surface of human B lymphoma lines. The cells were subsequently tested for their ability to absorb infectious EB virus. Stripping of Fc receptors, IgM