𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Supramolecular assemblies of adsorbed collagen affect the adhesion of endothelial cells

✍ Scribed by Z. Keresztes; P. G. Rouxhet; C. Remacle; C. Dupont-Gillain

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
645 KB
Volume
76A
Category
Article
ISSN
1549-3296

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The behavior of endothelial cells (HUVECs) in contact with thin collagen films presenting different supramolecular organizations was investigated. Collagen was adsorbed on polystyrene (PS) and plasma‐oxidized PS (PSox) in conditions ensuring the formation of continuous layers presenting an increasing density of fibrillar structures. Discontinuous collagen layers were also prepared on PS by adsorption followed by dewetting. The morphology of the obtained collagen films was checked by using atomic force microscopy. HUVECs adhesion was evaluated in terms of cell number, cell area, cell shape, and actin structure after 4 h of contact with the prepared collagen layers. In the presence of serum, no adhesion was observed on PS, whereas a substantial adhesion was found on PSox. This is explained by the competition for adsorption, which turns in favor of adhesive proteins secreted by the cells on the hydrophilic PSox, but turns in favor of serum albumin on the hydrophobic PS. The progressive coating of PS by smooth collagen films increased cell adhesion and spreading. However, cell spreading and cytoskeleton organization were adversely affected by the appearance of a high density of collagen fibrillar structures. This latter trend was similarly observed on PSox. On the other hand, HUVECs spreading and cytoskeleton organization were clearly enhanced on discontinuous collagen layers compared with continuous ones. A possible explanation for these observations lies in the modification of exposure and/or spatial distribution of recognition sequences due to spontaneous collagen self‐assembly on fibril formation or to collagen aggregation on dewetting. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006

📜 SIMILAR VOLUMES

Primary sequence of ionic self-assemblin
Primary sequence of ionic self-assembling peptide gels affects endothelial cell adhesion and capillary morphogenesis
✍ A. L. Sieminski; C. E. Semino; H. Gong; R. D. Kamm 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 411 KB

## Abstract Appropriate choice of biomaterial supports is critical for the study of capillary morphogenesis __in vitro__ as well as to support vascularization of engineered tissues __invivo.__ Self‐assembling peptides are a class of synthetic, ionic, oligopeptides that spontaneously assemble into g

Nanostructuration of titania films prepa
Nanostructuration of titania films prepared by self-assembly to affect cell adhesion
✍ John D. Bass; Emmanuel Belamie; David Grosso; Cédric Boissiere; Thibaud Coradin; 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 559 KB

## Abstract Nanostructured and dense titania films prepared by evaporation‐induced self‐assembly (EISA) are shown to possess tunable topographical nanoscale features on the order 2–12 nm. Thermal treatment (calcination) induces a transition from amorphous titania to crystalline anatase that modifie

A rough morphology of the adsorbed fibro
A rough morphology of the adsorbed fibronectin layer favors adhesion of neuronal cells
✍ C. M. Dekeyser; E. Zuyderhoff; R. E. Giuliano; H. J. Federoff; Ch. C. Dupont-Gil 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 487 KB

## Abstract A range of substrates made of polystyrene (PS) and poly(methyl methacrylate)–poly(methacrylic acid) copolymer (PMMA–PMAA) containing 98 and 80% PMMA (PA98, PA80) and presenting a homogeneous or a patterned surface were used to study fibronectin adsorption and neuronal cell behavior. Fib

Adhesion and growth of endothelial cell
Adhesion and growth of endothelial cell on amphiphilic PU/PS IPN surface: Effect of amphiphilic balance and immobilized collagen
✍ Kim, J. H. ;Kim, S. H. ;Kim, H. K. ;Akaike, T. ;Kim, S. C. 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 275 KB 👁 1 views

## Abstract Since natural blood vessels are lined with an endothelial cell (EC) monolayer, it is proposed that synthetic biomaterial surfaces be covered or seeded with endothelial cells for ideal nonthrombogenicity under normal conditions. The effects of the surface energy of hydrophilic‐hydrophobi

Effects of catechins on the mouse lung c
Effects of catechins on the mouse lung carcinoma cell adhesion to the endothelial cells.
✍ Mamoru Isemura; Yasuo Suzuki; Ken Satoh; Koh Narumi; Masakichi Motomiya 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 451 KB

## Abstract We studied the effects of 5 kinds of catechins on the adhesion of mouse lung carcinoma 3LL cells to the monolayer of bovine lung endothelial cells. (‐)‐Epicatechin gallate and (‐)‐epigallocatechin gallate were active in inhibiting the 3LL cell adhesion, while (+)‐catechin and (‐)‐epicat