Suppression of proline-directed protein kinase FA systemically inhibits the growth of human acute leukemia cells

✍ Scribed by Chih-Ping Hsu; Chuan-Ching Yang; Shiaw-Der Yang

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: French
• Weight: 100 KB
• Volume: 91
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/1097-0215(200002)9999:9999<::aid-ijc1100>3.0.co;2-3

✦ Synopsis

Initial studies revealed that proline-directed protein kinase F A (PDPK F A ) was overexpressed in various cancerous tissues relative to normal controls. However, the functional role of overexpressed PDPK F A in cancer remains to be established. In this report, we explore the potential role of PDPK F A in leukemia cell growth by investigating the effects of partial inhibition of this kinase on human acute promyelocytic leukemia (HL-60) and acute lymphoblastic leukemia (Jurkat) cells. Cloning of PDPK F A cDNA and its recombinant antisense expression vector and antibody were successfully developed. Several stable antisense clones of HL-60 and Jurkat cells were subcloned, which expressed a low level of PDPK F A when compared with the control-transfected clone in immunoblot analysis. Moreover, these antisense clones potently inhibited cell growth, clonogenic growth in soft agar and serum-independent growth. The results taken together demonstrate that suppression of PDPK F A is able to interfere with the growth of HL-60 and Jurkat cells, suggesting an essential role of this PDPK in human acute leukemia cell growth.