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Suppression of arthritis by an active center analogue of Cu2Zn2-superoxide dismutase

✍ Scribed by R. Miesel; A. Dietrich; B. Brandl; N. Ulbrich; M. Kurpisz; H. Kröger


Publisher
Springer
Year
1994
Tongue
English
Weight
787 KB
Volume
14
Category
Article
ISSN
0172-8172

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An investigation of Cu2Zn2superoxide dis
✍ L. Banci; I. Bertini; P. Turano 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 554 KB

The activity profile of the Cu2Zn2HSOD Ile-137 mutant has a pKa of 9.6, i.e. one unit lower than the wild type (WT). This property has allowed us to investigate the inactive high pH form of the enzyme before denaturation occurs. The electronic and EPR spectra do not change with the above pKa. The 1H