Abstract
The polyphenols determined are: (+)‐catechin, (–)‐epicatechin, rutin, quercetin and trans‐resveratrol. Suitable conditions of supercritical fluid extraction were established using ethanol as a modifier of the polarity solvent (supercritical carbon dioxide). Final extraction conditions were: 20% v/v ethanol, 60°C, 250 bars and flow rate 2 mL/min. Static step time and dynamic step time were established using a selected grape skin sample. The extract was collected in water; the more polar polyphenols ((+)‐catechin and (–)‐epicatechin) remain in solution but rutin, quercetin and trans‐resveratrol precipitate in this medium, thereby the solution of the extracted polyphenols was filtered. (+)‐Catechin and (–)‐epicatechin were determined in the liquid fraction, while the solid fraction, containing rutin, quercetin and trans‐resveratrol, was solved with ethanol/H~2~O (40:60). HPLC determination was carried out at C~18~ stationary phase, with ethanol/water/acetic acid as mobile phases and UV‐visible diode array detection. Due to the significant differences between the polarity of the polyphenols, two different mobile phases were used. An ethanol/water/acetic acid (5:93:2) mobile phase was used to determine (+)‐catechin (280 nm) and (–)‐epicatechin (280 nm). On the other hand, rutin (254 nm), quercetin (254 nm) and trans‐resveratrol (306 nm) were resolved using ethanol/water/acetic acid (40:58:2) as mobile phase. Instrumental parameters were optimised and analytical parameters obtained. The analytical method was validated and applied to five different varieties of Vitis vinifera from the geographical area of Valencia.