Sulfo-N-hydroxysuccinimide interferes with bicinchoninic acid protein assay
โ Scribed by Sandeep Kumar Vashist; BinBin Zhang; Dan Zheng; Khalid Al-Rubeaan; John H.T. Luong; Fwu-Shan Sheu
- Book ID
- 113412123
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 402 KB
- Volume
- 417
- Category
- Article
- ISSN
- 0003-2697
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The interference of a range of drugs and related substances has been investigated in the Bradford Coomassie brilliant blue (CBB) protein dye-binding assay and the 2,2'-bicinchoninic acid (BCA) protein assays. Chlorpromazine was the only substance to interfere in the CBB assay but the interference wa
At a concentration of 20-800 nmol/0.1 ml hydrogen peroxide instantly reacts with a 2,2'-bicinchoninic acid copper color reagent. It also reacts with a reformulated reagent at pH 7 but the color develops less rapidly. While the effect may interfere with protein estimations at alkaline pH, the effect
The bicinchoninic acid (BCA) assay method for the determination of protein has been investigated for its utility in measuring the protein content of plasma lipoproteins. Although other methods, principally those based on the method of Lowry et al. (1951, J. Biol. Chem. 193, 265-275) have been extens
The use of bicinchoninic acid (BCA) to measure protein concentrations has received wide acceptance because the reagent is insensitive to many of the buffers, sucrose solutions and detergents used with various tissue and enzyme preparations. However, any compound capable of reducing Cu2+ in an alkali