𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Drug interference in the Bradford and 2,2′-bicinchoninic acid protein assays

✍ Scribed by Thomas Marshall; Katherine M. Williams

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
270 KB
Volume
198
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

The interference of a range of drugs and related substances has been investigated in the Bradford Coomassie brilliant blue (CBB) protein dye-binding assay and the 2,2'-bicinchoninic acid (BCA) protein assays. Chlorpromazine was the only substance to interfere in the CBB assay but the interference was slight. In contrast, the BCA reagent interacted strongly with chlorpromazine, the penicillins, vitamin C, and paracetamol and the mode of interference varied with the test substance. The chlorpromazine produced turbidity and an atypical color. The penicillins show a slow but normal color response while vitamin C and paracetamol gave an immediate and intense response.

📜 SIMILAR VOLUMES

Potential interference of hydrogen perox
Potential interference of hydrogen peroxide in the 2,2′-bicinchoninic acid protein assay
✍ W.L. Baker 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 221 KB

At a concentration of 20-800 nmol/0.1 ml hydrogen peroxide instantly reacts with a 2,2'-bicinchoninic acid copper color reagent. It also reacts with a reformulated reagent at pH 7 but the color develops less rapidly. While the effect may interfere with protein estimations at alkaline pH, the effect

Modification of the bicinchoninic acid p
Modification of the bicinchoninic acid protein assay to eliminate lipid interference in determining lipoprotein protein content
✍ Richard E. Morton; Tracy A. Evans 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 356 KB

The bicinchoninic acid (BCA) assay method for the determination of protein has been investigated for its utility in measuring the protein content of plasma lipoproteins. Although other methods, principally those based on the method of Lowry et al. (1951, J. Biol. Chem. 193, 265-275) have been extens

H2O2 as a DNA fragmenting agent in the a
H2O2 as a DNA fragmenting agent in the alkaline elution interstrand crosslinking and DNA-protein crosslinking assays
✍ Leszek Szmigiero; Kazimierz Studzian 📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 423 KB

A method for DNA fragmentation by H2O2 in the DNA alkaline elution procedure is described. Treatment of cell suspensions for 1 h with 100 microM H2O2 or 5 mM H2O2 at 0-1 degree C resulted in DNA breakage equivalent to doses of 300 and 3000 rad of gamma-rays, respectively. The elution profiles were r

Transport of ionizable drugs and protein
Transport of ionizable drugs and proteins in crosslinked poly(acrylic acid) and poly(acrylic acid-co-2-hydroxyethyl methacrylate) hydrogels. I. Polymer characterization
✍ Mary Tanya Am Ende; Nikolaos A. Peppas 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 874 KB

The purpose of this investigation was to define the polymer structure and elucidate the swelling behavior of ionizable hydrophilic polymers (hydrogels) in water and buffered media. Poly(acry1ic acid) (PAA) and poly(acry1ic acid-co-2-hydroxyethyl methacrylate) [P(AA-co-HEMA)] hydrogels were synthesiz

Mutagenicity and cytotoxicity of n-methy
Mutagenicity and cytotoxicity of n-methyl-2-pyrrolidinone and 4-(methylamino)butanoic acid in the Salmonella/microsome assay
✍ David A. Wells; Harvey F. Thomas; George A. Digenis 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 482 KB

The industrial solvent N-methyl-2-pyrrolidinone (NMP) and its hydrolysis product, 4-(methylamino)butanoic acid (N-MeGABA), were examined for mutagenicity and cytotoxicity in the Ames Salmonella/microsome assay. In order to detect a broad range of possible mutagenic endpoints, the following strains w

Use of 2,4,6-trinitrobenzenesulfonic aci
Use of 2,4,6-trinitrobenzenesulfonic acid for the coestimation of amines, amino acids, and proteins in mixtures
✍ Lewis C. Mokrasch 📂 Article 📅 1967 🏛 Elsevier Science 🌐 English ⚖ 414 KB

In the course of an investigation on the reaction between biogenic amines and proteolipids (1)) methods were required to analyze for proteins and amines in mixture. The method of Satake et al. ( 2) proved to have some easily corrected disadvantages and some unexploited potentialities which make it i