An acidic, partially O-acetylated O-specific polysaccharide was obtained by mild acid degradation of Shigella boydii type 5 lipopolysaccharide and studied by 1H and 13C NMR spectroscopy, including 2D COSY, 13C-1H heteronuclear COSY, 1D NOE, and 2D ROESY experiments, and chemical methods (sugar and m
Sudies of O-specific polysaccharide chains of Pseudomonas solanacearum lipopolysaccharides consisting of structually different repeating units.
โ Scribed by Nina A. Kocharova; Yuriy A. Knirel; Alexander S. Shashkov; Nikolay E. Nifant'ev; Nikolay K. Kochetkov; Lyudmila D. Varbanets; Natalya V. Moskalenko; Oksana S. Brovarskaya; Valentina A. Muras; John M. Young
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 813 KB
- Volume
- 250
- Category
- Article
- ISSN
- 0008-6215
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โฆ Synopsis
The structures of the 0-antigenic polysaccharide chains of lipopolysaccharides of a number of Pseudomonas solanaceanrm strains were elucidated mainly with the help of methylation analysis and 13C NMR spectroscopy, including a computer-assisted t3C NMR-based analysis. Six structurally distinct but related polysaccharides were identified. They have a backbone which is built up of three L-rhamnopyranosyl residues and one 2-acetamido-2-deoxy-p-ghrcopyranosyl residue, and is unsubstituted or substituted with a residue of L-xylopyranose or L-rhamnopyranose as a monosaccharide side chain. The lipopolysaccharides of most of the strains contain polysaccharide chains consisting of at least two structurally different types of repeating units. Three of the polysaccharides are common to more than one strain.
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The O-specific polysaccharide isolated by mild acid degradation of the lipopolysaccharide of Y. kristensenii strain 490 (O:12,25) contained D-glucose, 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, 2-acetamido-2,6-dideoxy-L-galactose, glycerol, and phosphate in the ratios 2:2:1:1:1:
In a study' of the lipopolysaccharides of Pseudomonas syringae and related species, the structure of the O-antigen of P. syringae pv. tabaci strain 223 belonging to serogroup VII has been established. We report now the structure of the O-specific polysaccharide of another representative of this path
The O-specific polysaccharide, liberated by mild acid hydrolysis of the lipopolysaccharide (LPS-P) from Thiobacillus sp. IFO14570 as isolated from the phenol phase after phenol-water extraction, is shown to have a linear tdsaccharide repeating-unit containing three different diamino sugars, namely 2