Sucrose interference in the assay of enzymes and protein
โ Scribed by R.H. Hinton; M.L.E. Burge; G.C. Hartman
- Book ID
- 107711024
- Publisher
- Elsevier Science
- Year
- 1969
- Tongue
- English
- Weight
- 542 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0003-2697
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Sucrose has been found to decrease the fluorescence observed during the standard fluorometric assay of tryptophan. The quenching is enhanced with increasing concentrations of sucrose. This phenomenon is not due to a metal contaminant in the sucrose, nor to a direct interaction of the sugar with the
The nonionic detergent Tween 80, which has been widely used to stimulate protein secretion in bacterial and fungal systems, caused interferences in three protein determination methods. The OD595 developed in the Coomassie blue dye-binding assay with a variety of purified proteins in the presence of