The substrate specificity factor, VcKo/ V o Kc, of spinach (Spinacia oleracea L.) ribulose 1,5-bisphosphate carboxylase/oxygenase was determined at ribulosebisphosphate concentrations between 0.63 and 200 gM, at pH values between 7.4 and 8.9, and at temperatures in the range of 5 ~ C to 40 ~ C. The
Subunit arrangement of spinach ribulose 1,5-bisphosphate carboxylase/oxygenase
β Scribed by J. A. Barcena; P. J. Shaw
- Publisher
- Springer-Verlag
- Year
- 1985
- Tongue
- English
- Weight
- 879 KB
- Volume
- 163
- Category
- Article
- ISSN
- 0032-0935
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β¦ Synopsis
The structure of spinach ribulose 1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) has been investigated by tilted-view electron microscopy of negatively stained monolayer crystals and image processing. The structure determined consists of a cylinder of octagonal cross-section with a large central hole. Based on this and other available evidence a model for the arrangement of the large and small subunits is suggested with the eight small subunits arranged equatorially around the core of eight large subunits.
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