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EPR studies of ribulose-1,5-bisphosphate carboxylase/oxygenase activated With Cu2+

✍ Scribed by Stenbjörn Styring; Rolf Braändén; Thomas Nilsson

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
200 KB
Volume
79
Category
Article
ISSN
0020-1693

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✦ Synopsis

NAD from the adsorbent loaded by Cu'+, Cd'+, Zn2+ (purification factor subsequently -15, 20, 17, yield -56-68%) but not Ni2+, Mn2+ (yield 2.2 and 15.8%) or Co'+, Fe3+ (not eluted). This study supports the idea that the interaction of such metal ions as Cu2+ and Zn2+ (Cd") with LRY chromophore (in contrast to the metal ions Mn2+ and Ni2+) stabilizes a particular conformation of dye that is sterically acceptable to the NAD-binding site in ADH.

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