Substrate-Dependent Mechanisms in the Catalysis of Human Immunodeficiency Virus Protease
β Scribed by Polgar, Laszlo; Szeltner, Zoltan; Boros, Imre
- Book ID
- 126882296
- Publisher
- American Chemical Society
- Year
- 1994
- Tongue
- English
- Weight
- 781 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0006-2960
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## Peptidomimetic Human The human immunodeficiency virus (HIV) codas f o r an u.spartic protease known to be essential $)r retroviral mat urution and replicution. The H I V proteuw can rmignize Phe-Pro and Tyr-Pro sequences as the virus-specific cleuvuge site. These.fi.atures provided a basisJor th
A solid phase assay for human immunodeficiency virus (HIV) protease using an immobilized substrate, Affi Gel 10-Gly-Gly-Gly-Gly-Val-Ser-Gln-Asn-Tyr-Pro-Ile-Val-Gln-[3H]Gly-OH has been devised. The Tyr-Pro bond of the substrate was hydrolyzed by the protease, releasing the radiolabeled cleavage produ