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Substrate concentration control by dialysis in fermentations of Escherichia coli

✍ Scribed by B. G. Thompson

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
671 KB
Volume
28
Category
Article
ISSN
0006-3592

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A method was developed to provide a real-time measurement of intracellular adenosine 5'-triphosophate (ATP) concentrations in growing Escherichia coli. The bacteria to be monitored must first be modified by inserting the cDNA for firefly luciferase expressed from a constitutive promoter. Such a cons