Fermentation conditions for high-level expression of the tac-promoter-controlled calf prochymosin cDNA in Escherichia coli HB101

High-level expression of lacZ under cont

High-level expression of lacZ under control of the tac or trp promoter using runaway replication vectors in Escherichia coli

✍ John Kidwell; Dana Kolibachuk; Douglas Dennis 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 738 KB

To determine the utility of coupling runaway replication to the expression of cloned genes under the control of strong promoters, lacZ transcriptional fusions to the trp or tac promoter (Ptrp or Ptac) were constructed using plasmids in which the copy number is thermally regulated. Cells containing t