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Substitution patterns in alleles of immunoglobulin V genes in humans and mice

✍ Scribed by Tania Romo-González; Enrique Vargas-Madrazo


Book ID
116755602
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
579 KB
Volume
43
Category
Article
ISSN
0161-5890

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Immunoglobulin constant kappa gene allel
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Genomic DNA for the immunoglobulin (Ig) constant kappa Igk-C gene region was amplified by the polymerase chain reaction (PCR) method and sequenced from twelve commonly used inbred mouse strains. PCR products were used directly as templates in dideoxy-DNA-sequencing, a method which avoids the sequenc