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Novel Substitution Polymorphisms of Human Immunoglobulin VH Genes in Mexicans

✍ Scribed by Tania Romo-González; Jorge Morales-Montor; Mauricio Rodríguez-Dorantes; Enrique Vargas-Madrazo


Book ID
116530736
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
443 KB
Volume
66
Category
Article
ISSN
0198-8859

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## Polymerase chain reaction 2 Materials and methods ### 2.1 Cell isolation and DNA extraction Granulocytes were isolated from peripheral blood of two unrelated individuals, A and B, on a Ficoll-Paque (G-S Chemical MFG. Corp.) gradient. High-molecular weight genomic DNA was extracted from the gr