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Substance P immunoreactivity of rat brain stem neurons in primary culture

✍ Scribed by Dr. T. D. Jacquin; J. P. Denizot; M. Denavit-Saubie

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 466 KB
Volume: 31
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490310118

No coin nor oath required. For personal study only.

✦ Synopsis

Substance P (SP)-ergic neurons from 16/17 day-embryonic rat brain stem in primary culture were identified by immunocytochemistry using biotinylated avidine and phosphatase alcaline methods with affinity-purified anti-SP antibodies. An average of 84% of neurons contained SP from day 9 to day 21 after plating. These in vitro data show that SP-containing neurons develop in our culture conditions. SP may act as a maturation factor as well as a neurotransmitter.

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