Submerged growth of cells of higher plants

✍ Scribed by Nickell, Louis G. ;Tulecke, Walter

• Publisher: Wiley (John Wiley & Sons)
• Year: 1960
• Weight: 730 KB
• Volume: 2
• Category: Article
• ISSN: 0368-1467
• DOI: 10.1002/jbmte.390020305

✦ Synopsis

The submerged propagation of plant tissue and cell cultures has now reached the stage where mass amounts of material can be produced. In discussing these advances, various aspects of the technology involved are discussed: the methods of isolation and establishment of cultures, the media used for culture and their effects on growth, the systems used for growth and a comparison of their effectiveness.

Biochemical differences are shown between cultures grown under in vitro conditions and the organ from which they were derived. These differences are found among amino acids, organic acids, nucleic acids, and sugars. The potential use of the tissue culture approach for the study of the biosynthesis of 'secondary compounds' such as alkaloids and steroids is discussed. * Presented at the Symposium on 'Mass Propagation of Cells', sponsored by the Fermentation Subdivision, Division of Agricultural and Food Chemistry, 136th