Isolation of protoplasts from higher plant cells
โ Scribed by P. G. Pinto da Silva
- Publisher
- Springer
- Year
- 1969
- Tongue
- English
- Weight
- 300 KB
- Volume
- 56
- Category
- Article
- ISSN
- 0028-1042
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๐ SIMILAR VOLUMES
A protocol has been established for rapid, high frequency plant regeneration from protoplasts of the wild tomato species Lycopersicon chilense Dun. Cell suspension cultures were obtained from calli initiated from seedling stem explants. Protoplasts were isolated from cell suspensions by an overnight
Yields of 106-108 peach mesophyll cells and protoplasts-gfw -1 were obtained depending on factors such as digesting enzymes, and leaf size. Onozuka R-10 (2%) in combination with Macerase (0.5%) was found best for protoplast isolation and mediocre for cell isolation among several enzyme combinations