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Subdiffraction resolution in far-field fluorescence microscopy

✍ Scribed by Klar, Thomas A.; Hell, Stefan W.


Book ID
115423627
Publisher
Optical Society of America
Year
1999
Tongue
English
Weight
892 KB
Volume
24
Category
Article
ISSN
0146-9592

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## Abstract Subdiffraction fluorescence imaging is presented in a parallelized wide‐field arrangement exploiting the principle of reversible saturable/switchable optical transitions (RESOLFT). The diffraction barrier is overcome by photoswitching ensembles of the label protein asFP595 between a non