Subcellular localization of neural-specific NPDC-1 protein

✍ Scribed by C. Evrard; P. Rouget

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 871 KB
• Volume: 79
• Category: Article
• ISSN: 0360-4012
• DOI: 10.1002/jnr.20405

✦ Synopsis

Abstract

NPDC‐1 is a gene specifically expressed in neural cells when they stop to divide and begin to differentiate. Immunocytochemical study analysis of differentiated PC12 cells transfected with NPDC‐tag vectors showed that NPDC‐1 is transported in vesicles from the Golgi apparatus to the cell membrane and is then likely internalized into endosomes. The protein colocalized, at least partially, with synaptic vesicle proteins: synaptophysin, synaptobrevin 2, and Rab3 GEP (Rab3 GTP/GDP exchange protein). Moreover, subcellular fractionation of rat brain showed that crude synaptic membrane and crude synaptic vesicle fractions were enriched in NPDC‐1. Although NPDC‐1 bound Rab3 GEP in vitro, it seems unlikely to be involved in Ca^2+^‐dependent exocytosis and, thus, in synaptic vesicle trafficking. © 2005 Wiley‐Liss, Inc.