Subcellular localization of G-proteins in primary-cultured mouse preadipocytes and adipocytes

✍ Scribed by Nicole Bégin-Heick; Marsha A. Black; Johanna Gaffield; Monique Cadrin

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 323 KB
• Volume: 65
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/(sici)1097-4644(199705)65:2<259::aid-jcb11>3.0.co;2-d

✦ Synopsis

The subcellular localization of G s a, G i a1&2, G i a3, and Gb was studied in primary-cultured undifferentiated and differentiated, lipid replete, adipose cells. The results show a distinct distribution for each of these G-proteins and differences between differentiated and undifferentiated cells. All the G-proteins examined had a cytoplasmic localization; only G i a1 and 2 showed a significant colocalization with the plasma membrane and this only in differentiated cells. Most studies using cells in culture have reported an intracellular localization for G-proteins, whereas in tissue sections the localization has been reported to be largely with the plasma membrane, with some intracellular localization. The results suggest that the cell-cell interactions or the specific geometry imposed by culture conditions favor the intracellular compared to peripheral localization of G-proteins. Alternately, the posttranslational modifications necessary for G-protein insertion in the plasma membrane may be deficient in cultured cells.