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Comparison of the subcellular distribution of G-proteins in hepatocytes in situ and in primary cultures

✍ Scribed by Monique Cadrin; Norma McFarlane-Anderson; Mary-Ellen Harper; Johanna Gaffield; Nicole Bégin-Heick

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Volume
62
Category
Article
ISSN
0730-2312

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✦ Synopsis

The subcellular localization of the heterotrimeric G-proteins in hepatocytes in situ was compared to that in hepatocytes in primary culture. The ability of various ligands to activate adenylyl cyclase (AC) in membrane preparations was also investigated. In hepatocytes in situ the G proteins were mainly localized at the plasma membrane while in hepatocytes in culture they were predominantly cytoplasmic. The localization of the G-proteins in hepatocytes in situ correlates with their role in signal transduction. In homogenates prepared from the cultured tells, ligands which stimulate AC via G,a were without effect, which was consistent with the localization of G,a in the cytoplasmic and nuclear compartments. The "relocalization" of the G proteins to the cytoplasm when cells are cultured suggests that transmembrane signalling may be regulated by cell differentiation and cell-cell and cell-extracellular matrix interactions.

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